Lipidomic profiling of bioactive lipids by mass spectrometry during microbial infections
نویسندگان
چکیده
منابع مشابه
Tissue Profiling by Mass Spectrometry
Matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) has become a valuable tool to address a broad range of questions in many areas of biomedical research. One such application allows spectra to be obtained directly from intact tissues, termed “profiling” (low resolution) and “imaging” (high resolution). In light of the fact that MALDI tissue profiling allows over a thousand...
متن کاملSensitive profiling of chemically diverse bioactive lipids.
Here, we present an improved method for sensitive profiling of lipids in a single high-performance liquid chromatography-electrospray ionization-quadrupole time of flight mass spectrometry experiment. The approach consists of i) sensitive isocratic elution, which takes advantage of C18 column material that is resistant to increased pH values induced by piperidine, ii) chemometric alignment of m...
متن کاملLipidomic and Spatio-Temporal Imaging of Fat by Mass Spectrometry in Mice Duodenum during Lipid Digestion
Intestinal absorption of dietary fat is a complex process mediated by enterocytes leading to lipid assembly and secretion of circulating lipoproteins as chylomicrons, vLDL and intestinal HDL (iHDL). Understanding lipid digestion is of importance knowing the correlation between excessive fat absorption and atherosclerosis. By using time-of-flight secondary ion mass spectrometry (TOF-SIMS), we il...
متن کاملLipidomic profiling of biological tissues using off-line two-dimensional high-performance liquid chromatography-mass spectrometry.
Lipids are important components in all biological tissues having many essential roles associated with the proper function of the organism. Their analysis in the biological tissues and body fluids is a challenging task due to the extreme sample complexity of polar lipids and to their amphiphilic character. In this work, we describe a new method for the characterization of the lipid composition i...
متن کاملPrion protein allotype profiling by mass spectrometry*
Prion diseases or transmissible spongiform encephalopathies (TSEs) are fatal neurodegenerative pathologies characterized by the formation in the central nervous system of the amyloid protein PrPSc, which derives from a cellular precursor called PrPc. Epidemiological and laboratory studies have shown that in species where the PrPc gene is polymorphic, the genotype composition is an important fac...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Seminars in Immunology
سال: 2013
ISSN: 1044-5323
DOI: 10.1016/j.smim.2013.08.006